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Effect of α-MMC on proliferation of A549 and 95-D cells (A) . The cell activity of A549 and 95-D cells treated with α-MMC for 24 h, 48 h and 72 h was measured by cck-8 kit; (B) The half-inhibitory concentration (IC50) was analyzed based on α-MMC intervention in A549 and 95-D cells for 48 h; (C) When α-MMC interfered with A549 and 95-D cells for 48 h, the cell morphology was observed by phase contrast microscopy (×100); (D) The colony formation ability of A549 and 95-D cells was observed after 14 days of treatment with different concentrations of α-MMC.

Journal: Frontiers in Pharmacology

Article Title: The type I ribosome-inactivating protein α-MMC induced significant apoptosis of lung cancer A549 and 95-D cells by activating the caspase cascade through TNF signaling pathway

doi: 10.3389/fphar.2025.1529151

Figure Lengend Snippet: Effect of α-MMC on proliferation of A549 and 95-D cells (A) . The cell activity of A549 and 95-D cells treated with α-MMC for 24 h, 48 h and 72 h was measured by cck-8 kit; (B) The half-inhibitory concentration (IC50) was analyzed based on α-MMC intervention in A549 and 95-D cells for 48 h; (C) When α-MMC interfered with A549 and 95-D cells for 48 h, the cell morphology was observed by phase contrast microscopy (×100); (D) The colony formation ability of A549 and 95-D cells was observed after 14 days of treatment with different concentrations of α-MMC.

Article Snippet: The effect of α-MMC on morphological changes of A549 and 95-D cells was analyzed by phase contrast microscopy (OLYMPUS BX63, shanghai, China).

Techniques: Activity Assay, CCK-8 Assay, Concentration Assay, Microscopy

Effect of α-MMC on mitochondrial membrane potential in A549 and 95-D cells. α-MMC interfered with A549 cells for 72 h and 95-D cells for 48 h, respectively. (A) Cell ROS levels were measured by flow cytometry. (B) The changes of MMP after JC-1 staining were observed by fluorescence microscopy. (C,D) MMP was analyzed by flow cytometry and displayed in the histogram.

Journal: Frontiers in Pharmacology

Article Title: The type I ribosome-inactivating protein α-MMC induced significant apoptosis of lung cancer A549 and 95-D cells by activating the caspase cascade through TNF signaling pathway

doi: 10.3389/fphar.2025.1529151

Figure Lengend Snippet: Effect of α-MMC on mitochondrial membrane potential in A549 and 95-D cells. α-MMC interfered with A549 cells for 72 h and 95-D cells for 48 h, respectively. (A) Cell ROS levels were measured by flow cytometry. (B) The changes of MMP after JC-1 staining were observed by fluorescence microscopy. (C,D) MMP was analyzed by flow cytometry and displayed in the histogram.

Article Snippet: The effect of α-MMC on morphological changes of A549 and 95-D cells was analyzed by phase contrast microscopy (OLYMPUS BX63, shanghai, China).

Techniques: Membrane, Flow Cytometry, Staining, Fluorescence, Microscopy